Reverse osmosis removes healthy, naturally occurring water minerals. Often the only difference is the added minerals which have no proven health benefits. A four-year study by the Natural Resources Defense Council shows that 1/3 of the bottled water tested contains levels of contamination which exceed allowable limits. Genome Res. 2008, 18: 1689-1710.View ArticleGoogle ScholarChen WK, Swartz JD, Rush LJ, Alvarez CE: Mapping DNA structural variation in dogs. Nat Genet. 2007, 39: S30-S36. 10.1038/ng2042.PubMed CentralView Article. There are two distinct haplotypes that differ from each other by nearly 100 substitutions. One hundred and fifty-three probes on the array no longer had perfect sequence matches in the genome, 99.92% of the probes (715,130/715,698) had only a single perfect match and were therefore deemed to be single copies, and 568 probes had more than two perfect matches.
Protein coding genes that are related to important phenotypes were downloaded from Gramene Genes Database ( ) and then mapped onto the j-Npb genome. Genetics 145: 833–846.J. HeyJ. Wakeley1997A coalescent estimator of the population recombination rate.Genetics145833846 50. Saitou N, Nei M (1987) The neighbor-joining method: A new method for reconstructing phylogenetic trees. For each of the ten high divergence genes chosen for re-sequencing, we also carried out Southern analysis using genomic DNA of j-Npb, i-9311, and O. rufipogon digested with two different restriction enzymes (see Figure S3). In each case, only a single hybridizing restriction fragment was observed.
Genes in high divergence regions were tested for overrepresentation using complete gene annotations as reference and BiNGO . Genes for which there is no annotation are not taken into account in the overrepresentation analysis. The largest sequence length within the LINE signatures was 5540 bp. When singletons are excluded, the LD patterns of high polymorphism genes do not change. Importantly, some of the genes that reside in these regions of high polymorphism are likely to correspond to loci of biological significance.